Concerning the Wassermann Reaction
نویسنده
چکیده
tionally collected and kept for some time, is a very poor control. Some positive sera give a complete fixation of the usual one or more MHD of the complement in a 1 in 200 dilution; others can do so in 1 in 5 dilution only. The margin.of variation allowed for in this control, 5 to 200, is too wide to be useful in indicating whether the antigen-antibody systems involved in the reaction of complement fixation are working with the usual sensitiveness and specificity on a particular day. The writer pools and preserves sera fixing 4 MHD of complement with an uncholesterinized antigen (Greval, Das and Sen Gupta, 1938; Greval, Chandra and Das', 1940). A 1 in 50 dilution of the pooled sera gives a complete fixation and a 1 in 100 dilution an incomplete fixation with the usual cholesterinized antigen and the smaller dose of the complement. Sometimes the potency of the serum is greater. The potency is determined by titration. It keeps unchanged until the serum is exhausted and yields repeatable reactions, day after day, for months. It is pointed out that without the employment of titrated positive controls, the YTassermann reaction of borderline cases of syphilis is not repeatable and therefore not reliable. The writer is aware of the reported seasonal variability of the Wassermann reaction (Hoverson et al, 1935). He has, however, personal experience of the seasonal variability of the fixability of the complement. The latter variability must be excluded by the titration controls; otherwise an increase or decrease in the reaction of a serum may result from this cause alone; in fact the writer believes that more often than not it does so result. 2. The complement.?This reagent has six attributes which affect the reaction of fixation significantly. It may be (i) of high titre, (it) of low titre, (Hi) of optimal titre, (iv) cholesterol shy, (v) cholesterol fast, and (vi) of optimal reaction with cholesterol. The optimal titre is given by 1 in 40, 50 and
منابع مشابه
Concerning the Wassermann Reaction
Sir,?In the December 1943 issue of the Indian Medical Gazette under the article on ' Concerning the ?Wassermann Reaction' I fail to see why smallpox vaccination as one of the causes producing false positive reactions has not been mentioned. Hence will you please publish the following in your valuable monthly :? False positive syphilitic reactions have been attributed to numerous causes, with sm...
متن کاملPreliminary evaluation of the treponemal Wassermann reaction (T.W.R.).
Most of the relatively few papers on the treponemal complement-fixation reaction give good reports of this test. Thus, for example, Garson and Portnoy (1957) report that the treponemal complement-fixation test gives good reproducibility, is at least as sensitive in early syphilis as the conventional reagin tests, and has a greater value in confirming a diagnosis of early syphilis than the trepo...
متن کاملSerological Wassermann problem cases.
The subject of Wassermann "Problem" cases is of interest not only to the serologist but to the physician or clinician, upon whom devolves the responsibility for diagnosis. In the past there has been too little critical judgment by clinicians in assessing the results of the Wassermann reaction, and many practitioners may still regard a positive Wassermann reaction as in itself a diagnosis of syp...
متن کاملComplement Deviation in Scarlet Fever with Comparative Studies of the Wassermann and Noguchi Systems
The Wassermann reaction in scarlet fever per se is uniformly negative. The antigen of scarlet fever liver yields practically the same results as that of luetic liver, and both fail to deviate complement with scarlet fever antibodies. The Noguchi reaction in scarlet fever is practically negative. Sixteen cases, or 6.4 per cent. of 250 cases, were positive when active serum was used; with inactiv...
متن کاملthe Butyric Acid Test for Syphilis in the Diagnosis of Metasyphilitic and Other Nervous Disorders
1. In the secondary and tertiary stages of syphilis without direct involvement of the nervous system the cerebro-spinal fluid yielded the butyric acid reaction of feeble intensity. The fluid under these conditions gave neither a positive cytodiagnosis nor the Wassermann test for syphilis. 2. The cerebro-spinal fluid of cases of hereditary syphilis showed a positive butyric reaction in about 90 ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 78 شماره
صفحات -
تاریخ انتشار 1943